منابع مشابه
Kinetics of cell death and disintegration in human lymphocyte cultures.
In order to quantitate lymphocyte proliferative responses, we explored the role of cell death in the kinetics of phytohemagglutinin-stimulated cultures. Unless the disintegration time (tDIS) of nonviable lymphocytes in culture is known, the rate of cell death cannot be calculated. To obtain tDIS, we determined the time interval between total and viable cell population decay after various killin...
متن کاملStimulated Lymphocyte Cultures
Lymphocytes, stimulated with different doses of plant mitogens or allogeneic cells, incorporate varying amounts of [(3)H]thymidine. Theoretically, this may be due to different numbers of responding cells, to earlier proliferative response of these cells, and/or to their more or less rapid transit through the cell cycle. Dog peripheral blood lymphocytes were stimulated in vitro with different do...
متن کاملEffect of low molecular weight human serum factors and human somatomedin peptides on human lymphocyte cultures.
Using PH]thymidine incorporation into DNA of human lymphocytes in culture, we have shown that human serum contains an ultrafiltrable factor(s) that sensitizes the activation of lymphocytes by phytohaemagglutinin. This (these) factor(s) can replace either human serum or foetal calf serum in the culture. In addition, it permits the expression, and therefore the assay, of human somatomedin A pepti...
متن کاملRadioadaptive Response in Human Lymphocyte Cells
The adaptive response (AR) is a phenomenon by which cells exposure to sublethal doses of DNA-damaging agents (non-mutagenic dose of chemical or radiation), known as conditioning treatment (CT), leads to increased resistance to a subsequent exposure to a higher dose of the same or other agents, known as challenge treatment (CR). The adaptive response (AR) induced by radiation in human lymphocyte...
متن کاملHuman tonsillar tissue block cultures differ from autologous tonsillar cell suspension cultures in lymphocyte subset activation and cytokine gene expression.
Lymphoid tissues cultured either as tissue blocks or as cell suspensions are used to study the behaviour of immune cells within their habitat. The preservation of tissue structures in tissue blocks, which is considered to be a major advantage, has been poorly defined. We characterised the morphological evolution of tissue cultures from human palatine tonsils and compared their lymphocyte subset...
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ژورنال
عنوان ژورنال: European Journal of Cancer (1965)
سال: 1977
ISSN: 0014-2964
DOI: 10.1016/0014-2964(77)90104-9